Liquid Culture Kit Instructions

  1. This work can be done in a clean room with doors, windows, and air conditioning closed. There must be no air movement. Alternatively, it can be done inside a SAB—a large, clear, inverted plastic box.
  2. Wear gloves, mask and clean the work area thoroughly with 70% alcohol solution
  3. Remove the film from the syringe and jar
  4. Thoroughly clean the syringe, jar, and new, packaged needle with a 70% alcohol solution. Pay particular attention to the black injection port on the jar. NEVER open the jar; leave the cap as is!
  5. Open the sterile needle's paper wrapper and simultaneously remove the coloured cap from the syringe. Be careful not to pull off the clear tip on which the cap is attached . Immediately attach the needle to the syringe tip.
  6. Breathe. Now remove the plastic needle cap, being careful not to completely detach the needle from the syringe , and insert the needle into the black injection port on the jar. Breathe.
  7. Inject all the liquid into the jar slowly. Very slowly, remove the syringe from the injection port.
  8. DONE! Your LC is now ready to be inoculated with liquid culture mycelium! If you have a liquid culture syringe from us, the procedure will be the same! The only difference is that the needle will be screwed onto the syringe instead of stuck.
  9. INCUBATION PHASE : Place the jar in a clean place at a temperature between 20-27C
  10. After a week, stir your liquid culture in the jar to break up the mycelium and prevent it from forming lumps. To do this, gently rock the jar to create a circular motion within the liquid. CAUTION : Don't get the filter on the lid wet with the liquid inside the jar! Stir gently, avoiding splashes!