FAQ - Sterile substrates

How long does sterilized oats last?

Bags of sterilized oats can last up to two months. Despite this, we recommend not waiting more than a couple of weeks before inoculating. 

 

How should I store the sterile substrates? 

Sterile substrates should be kept in a clean place at room temperature and away from direct sunlight. The bags should be kept upright, with the filter at the top. Make sure that no substrate or grain is in contact with the filter, and that there is a good distance between the two. Having organic material in contact with the filter promotes the entry of contaminants through the filter.

 

How much liquid culture / spore solution should I inject into the bag of oats?

Bastano 2-4ml of solution. If using liquid culture, you can also add 10ml to accelerate colonization. Make sure to test your liquid culture or spore solution on agar to verify that it is free of contaminants. The sterility of our liquid cultures is guaranteed!

 

After inoculation, how should I store the substrates?

Store the inoculated substrates and grains upright with the filter at the top, at temperatures of around 25C, away from direct sunlight, preferably in the dark.

 

How long does it take before my substrate is completely colonized?

Colonization times depend on many factors. In particular, growth will be slower at colder temperatures and faster at higher temperatures. The speed of colonization depends mainly on the species and variety being cultivated.