2. Inoculation
Inoculate 5ml of liquid culture by injecting the injection port with the sterile needle of your syringe.
If added
Inoculate 5ml of liquid culture by injecting the injection port with the sterile needle of your syringe.
Place the kit in a dark, clean space with a temperature between 20-27°C (68-81°F). You'll usually need to wait two weeks before seeing growth, but this may vary depending on the species you're growing. Be patient!
Once the mycelium has colonized 20-30% of the substrate, carefully (without damaging the bag) pinch the bag from the front and back just below the filter and gently pull it from the sides to allow air to enter through the filter. This will give you the necessary space for mixing.
Gently press down on the substrate, breaking it up, and remix it, redistributing the colonized portions. Firm the substrate back into its original shape, smoothing the surface thoroughly. Let it rest until fully colonized.